Pharmaceutical Analysis

Ph.D. study

  1. General Information
  2. Entrance Examination
  3. Framework Study Plan
  4. Human Resources
  5. Research Groups

1. General Information

  • Form of study:

full-time / combined

  • Language of study:

Czech / English

  • Standard length of study:

4 years

  • Form of entrance examination:


  • Validity of accreditation:

until 22nd May 2029

  • Education field:

Pharmacy (100 %)

Study Program Characteristics

The doctoral program of Pharmaceutical Analysis is focused on the analytical evaluation of drugs, bioanalytical studies and the development of new analytical methods for the evaluation of drugs. Graduate students in this program broaden their basic theoretical knowledge of pharmaceutical analysis and obtain professional experience upon working with state-of-the-art analytical equipment. Graduates are experts at all aspects of drug analysis – the development of new analytical methods, the validation of analytical methods, drug stability testing and their bioanalytical monitoring. They will be well qualified to work in analytical laboratories of pharmaceutical companies and research institutes, in clinical laboratories of hospitals as well as in the laboratories engaged in pharmacopoeial drug evaluation.    

Information on Graduate Employment

Graduates are experts in drug analysis - development of new analytical methodologies, validation of analytical methods, stability studies and bioanalytical drug monitoring. Graduates can work in control laboratories dealing with pharmacopoeial assessment of drugs, clinical laboratories in hospitals, control laboratories of pharmaceutical companies and research institutes.     

2. Entrance Examination

Framework Content of the Entrance Examination

Oral exam from the instrumental methods used in qualitative and quantitative analysis, the basic knowledge of chemical analysis, pharmacopoeial analytical methods for evaluation of drugs.

English language exam for English language knowledge assessment (C1 at least).

Conditions of Admission

  1. Admission to Doctoral studies is conditioned by successful completion of a Master´s study programme.
  2. Successful passed the entrance exam.

Additional Conditions for Bonus Points (e.g. Practice)

  • Successfully completed university studies in pharmacy, chemical or natural science field (rated 2 points). 
  • The level of diploma thesis performed at analytical related workplace (rated 3 points). 
  • Indication of the supposed training center and the approval of a specific supervisor with the management of the planned doctoral project (rated max. 5 points).

Criteria for the Evaluation Exams

  1. Professional knowledge level – reviewed on answers to questions stated in General content of entrance exam (max. up to 10 points)
  2. Submitted doctoral project – evaluation of project complexity, modern methodological approaches to solution, and the continuity of the project on topics already discussed by the Subject Area Board (max. up to 10 points)

The minimum admission limit is 20 points.

The Dean decides to accept the applicant(s) who have fulfilled the conditions of the admission procedure and, according to the number of points achieved, ranked in the order corresponding to the predetermined number of accepted applicants for the particular program.


3. Framework Study Plan

Study obligations

The study duties of students of this doctoral study program include at least 2 partial examinations from the following list:

  • Selected analytical methods
  • Electromigration methods
  • Non-separation flow methods in pharmaceutical analysis
  • Analysis of substances of natural origin
  • General pharmaceutical chemistry
  • Bioanalytical evaluation of drugs
  • Chromatographic methods in pharmaceutical analysis
  • Development and validation of analytical methods for quality assurance of drugs
  • Analysis of biopharmaceuticals
  • Mass spectrometry

At the beginning of the study student consults with the supervisor selection of these partial examinations. Teaching takes the form of recommended lectures, seminars, consultations and self-study of recommended literature.
The curriculum also includes an English language exam. This exam is not included in the number of compulsory exams. Students can also enroll for courses from other fields according to their individual focus.

At the end it is necessary to pass the state doctoral examination Pharmaceutical Analysis.

Publication activity requirements

The doctoral student should be able to approach the research work, be able to design the experimental work, critically evaluate the results and present them in the form of a publication written in English and published in an impact factor journal. He/she should be able to present partial results at professional conferences (including foreign ones) in the form of posters, but also short oral presentations.
Student co-authorship on 2 publications in IF journals is required, of which in at least one work student is the first author. Alternatively, only one publication may be published in a journal with IF above 5 and/or in the first decile.

Requirements for completing internships

An integral part of the study in this doctoral study program is a foreign internship for a total length of at least three months. The selection of a foreign workplace (in very exceptional cases, a workplace in the Czech Republic with a unique focus) is made in cooperation with the supervisor and is usually a workplace with a similar research direction.

Other study obligations

Other activities include the involvement of doctoral students in undergraduate education, usually in the form of practical exercises.

Dissertation themes

  • Examples of successfully defended dissertations
    • The development of new methods of ultra-high performance supercritical fluid chromatography for pharmaceutical applications
    • Development of novel approaches to automated sample preparation for pharmaceutical and environmental analysis
    • Ultilization of LC-MS in bioanalysis of anthracyclines and potential cardioprotective compounds
    • Nano-fibers as a modern extraction sorbents for extraction of samples in chromatographic analysis
    • Analysis of drugs and potential drugs in biological material using liquid chromatography
  • Themes of defended dissertations - Thesis repository
  • List of current doctoral dissertation themes

State Doctoral Examination

Areas for the state doctoral examination:

  1. Identification of drugs based on physical constants, physicochemical methods and chemical reactions.
  2. Purity of drugs, evaluation of impurities by chemical reactions and instrumental methods.
  3. Chiral purity of drugs, methods for evaluation.
  4. Use of spectral methods in pharmaceutical analysis.
  5. Application of mass spectrometry in pharmaceutical analysis.
  6. Methods for quantitative analysis of active substances and pharmaceutical formulations.
  7. Separation analytical methods used for the analysis of drugs and pharmaceutical formulations.
  8. Development and validation of analytical method for analysis of drugs and pharmaceutical formulations.
  9. Stability of drugs and pharmaceutical formulations, factors affecting stability, kinetic characteristics of decomposition reactions, stability tests.
  10. Trends in instrumental analytical methods used in pharmaceutical analysis.
  11. Techniques of sample preparation before analysis of biological material, modern and conventional approach, perspectives of their use in pharmaceutical analysis.
  12. Analysis of drugs and endogenous substances in biological material.
  13. Instrumental analytical and immunoanalytical methods in pharmaceutical analysis.
  14. Analysis of biopharmaceuticals.
  15. Principles of work in the mode of good manufacturing practice and good laboratory practice.

4. Human Resources

Subject Area Board

Subject Area Board Chair and study program guarantor

  • doc. PharmDr. Hana Sklenářová, Ph.D.

Members of Subject Area Board

  • doc. Ing. Petr Česla, Ph.D.
  • prof. PharmDr. Martin Doležal, Ph.D.
  • doc. Ing. Miloš Hroch, Ph.D.
  • doc. PharmDr. Lucie Chocholoušová Havlíková, Ph.D.
  • prof. RNDr. Jiří Klimeš, CSc.
  • doc. PharmDr. Radim Kučera, Ph.D.
  • doc. Ing. Miroslav Lísa, Ph.D.
  • RNDr. Milan Maděra, Ph.D.
  • doc. PharmDr. Ludmila Matysová, Ph.D.
  • prof. RNDr. Peter Mikuš, Ph.D.
  • prof. PharmDr. Lucie Nováková, Ph.D.
  • doc. RNDr. Miroslav Polášek, CSc.
  • prof. RNDr. Petr Solich, CSc.
  • prof. RNDr. Dalibor Šatínský, Ph.D.
  • prof. Ing. Ivan Špánik, DrSc.
  • doc. PharmDr. Petra Štěrbová, Ph.D.

Other members of the State Doctoral Examination and Dissertation Defense Committees other than the members of the Subject Are Board

  • doc. PharmDr. Petr Chocholouš, Ph.D.
  • PharmDr. Petr Kastner, Ph.D.

Other supervisors other than the members of the Subject Are Board

  • PharmDr. Juraj Lenčo, Ph.D.

5. Research Groups

Research groups lead by supervisors of doctoral study program Pharmaceutical Analysis

Pharmaceutical-analytical evaluation of drugs and other biologically active substances

Analytical Chemistry Group
Group leader: prof. RNDr. Dalibor Šatínský, Ph.D.

Pharmaceutical Analysis Group
Group leader: doc. PharmDr. Radim Kučera, Ph.D.

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