Granty a projekty

Syntéza a modifikace povrchů sloužících jako templáty pro uspořádání lipidů kožní bariéry

Skin is the largest organ in the human body and protects it from excessive water loss while prevents the entrance of undesired substances, allergens and microbes. Skin’s outermost layer, stratum corneum (SC), holds the principal skin barrier. Human SC consists of flattened dead cells known as corneocytes, that are embedded in a hydrophobic lipid matrix. Corneocytes are having ceramides covalently attached to their surface that are forming the so called Corneocyte Lipid Envelope (CLE). The free SC lipid matrix is composed of ceramides, free fatty acids and cholesterol in an approximately 1:1:1 molar ratio. CLE has been suggested to function as a template for the specific arrangement of lipids in SC decades ago, but no experimental evidence has proven this hypothesis yet. The main goal of this project is to evaluate if immobilized ceramides on different surfaces can serve as template for the spontaneous assembly of SC lipids. Surface engineering of three various materials (i.e. PAMAM dendrimers, cellulose nanocrystals, and glass surfaces) will be employed to chemically decorate these materials with ceramide analogues. The lipid-decorated surfaces will be tested for their ability to mimic the function of CLE. Such CLE models will be an innovative approach leading to more complex in vitro models of the skin barrier. Such advanced models would help in detailed understanding and improved management of diseases with defective CLE. [GAUK1097746]

Číslo: GAUK262821

Období: 01.01.2021 - 31.12.2023

Poskytovatel: Univerzita Karlova - Grantová agentura UK

Hlavní řešitel: Velissari Panagiota - Farmaceutická fakulta v Hradci Králové

Celkový objem financí: 541 000,00 Kč

Interní řešitelé

Hlavní řešitel: Panagiota Velissari - Farmaceutická fakulta v Hradci Králové, FaF HK
Spoluřešitel: Irene Sagrafena - Farmaceutická fakulta v Hradci Králové, FaF HK
Spoluřešitel: Tomáš Havrišák - Farmaceutická fakulta v Hradci Králové, FaF HK
Spoluřešitel: PharmDr. Pavla Jančálková - Farmaceutická fakulta v Hradci Králové, FaF HK
Spoluřešitel: Mgr. Veronika Ondrejčeková - Farmaceutická fakulta v Hradci Králové, FaF HK
Spoluřešitel: Richard Župina - Farmaceutická fakulta v Hradci Králové, FaF HK
Spoluřešitel: Dr. Georgios Paraskevopoulos, Ph.D. - Univerzita Karlova,
Školitel: prof. PharmDr. Kateřina Vávrová, Ph.D. - Univerzita Karlova,

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