Special Methods of Instrumental Analysis

The aim of the subject is to deepen the knowledge of students in analytical chemistry and to prepare them for solving analytical problems with use of instrumental methods. Emphasis is laid on the ability of students to select optimal methods and appropriate procedures with regard to the nature of the analysed object and to realize the scope and limitations of instrumental methods. Practical training is realized in 3- to 4-membered groups of students.


  • Introduction to special methods of instrumental analysis, classification of methods, principles.
  • Atomic-absorption spectrophotometry, absorbing medium, resonance spectral lines, instrumentation, analytical applications. Atomic-fluorescence spectrometry.
  • Luminescence analysis, fluorescence, phosphorescence, chemiluminescence, emission and excitation spectra, fluorimeters, spectrofluorimeters, qualitative and quantitative applications.
  • Turbidimetry and nephelometry
  • High-frequency spectroscopic methods, nuclear magnetic resonance, NMR spectrum, analytical applications, mass spectrometry, mass spectrum, analytical applications.
  • Electromigration methods, electrophoretic mobility, principles of separation. Capillary isotachophoresis , capillary zone electrophoresis, instrumentation, analytical applications.
  • Isoelectric focusation, micellar electrokinetic chromatography. Capillary electrochromatography. Instrumentation, applications.
  • Ion-exchange chromatography. Ion-exchange phenomena, cation and anion exchangers, ion-exchange equilibria, amino acid analyzers, molecular sieves, gel-permeation chromatography.
  • Pulse voltammetric methods. Difference pulse polarography. Stripping voltammetry.
  • Automation of analytical procedures, titrators, types of analyzers and their applications.
  • Flow-injection analysis, sequential injection analysis, automation of analytical procedures, principles, instrumentation, applications.

Practical Training

  • Potentiometric determination with use of ion-selective electrodes and computer-aided method of two standard additions.
  • Stripping voltammetry – determination of traces of impurities (heavy metals).
  • Capillary isotachophoresis (ITP).
  • Capillary zone electrophoresis (CZE).
  • UV spectrophotometry – determination of constituents of multi-component mixtures with use of diode-array detector.
  • Fluorimetry. Polarimetry.
  • High-performance liquid chromatography (HPLC).
  • Sequential injection analysis (SIA).


  • Rücker G., Neugebauer M.,Willems G.G.: Instrumentelle pharmazeutische Analytik, WVG Stuttgart 2001.
  • Robinson J.W., Skelly Frame E.M., Frame II G.M.. – Undergraduate Instrumental Analysis, Marcel Dekker, New York, , 2005
  • Klouda P.: Moderní analytické metody, nakladatelství Pavel Klouda, Ostrava 2003

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