Molecular Biology

Molecular biology deals with the study of the cellular processes on molecular level. The main focus of this subject is description of heredity-related biological macromolecules (DNA, RNA, proteins), their mutual interactions and regulation of their function. Furthermore, the subject scope is extended towards explanation of the most important experimental molecular techniques, basics of clinical genetics and molecular diagnostics, as well as application of genetic engineering (recombinant DNA technology) in Medicine and Pharmacy.

Syllabus

Lectures

  • Model organisms in molecular biology
  • Structure and organisation of prokaryotic and eukaryotic genomes
  • DNA replication
  • Cell cycle, cell death, cell cycle disturbancies
  • Genetic mutations
  • Genetic repair, recombination
  • Structure and types of RNA
  • Transcription + posttranscriptional modifications of RNA
  • Genetic code, ribosomes, translation
  • Posttranslational modifications, protein degradation
  • Regulation of gene expression in prokaryotes
  • Regulation of gene expression in and eukaryotes
  • Isolation of nucleic acids
  • Overview of enzymes used in experimental molecular biology
  • Polymerase chain reaction and its modifivations
  • Gel electrophoresis
  • DNA sequencing
  • Hybridisation, blotting
  • Molecular cloning, methods of gene transfer
  • Hereditary diseases
  • Molecular diagnostics

Literature

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