The article "Reversed-Phase Liquid Chromatography of Peptides for Bottom-Up Proteomics: A Tutorial," published by Juraj Lenčo et al. in the Journal of Proteome Research (IF2021: 5.370), has become with more than 1800 views in just two weeks after its publication the most read article within the last 30 days in the renowned proteomic journal issued by the American Chemical Society.
The quality of the article was also highly acknowledged in the proteomics blog "News in Proteomics Research," written by respected commentator Ben C. Orsburn from Johns Hopkins University, as well as in the public discussion on Twitter.
The article was prepared by members of the STARSS project and featured foreign experts. It emphasizes the importance of reverse-phased liquid chromatography (RPLC) in typically used LC-MS proteomic analyses, where the chromatographic part frequently remains in the shadow of MS detection. Spread over 47 pages and 28 figures is a wealth of practical information on key aspects and the fundamentals of peptide separation using RPLC for bottom-up proteomic applications. Recommendations in the tutorial are based on the recent experimental proteomic data and evidence-based and experimentally proved parameters of RPLC aiming at the highest possible proteomic performance of the whole LC-MS method.
The authors' ambition was to highlight the importance of RPLC in LC-MS-based proteomics and to stimulate the interest of practitioners focused on proteomics in the fundamentals of chromatography to boost the performance of their RPLC methods and help them troubleshoot issues often encountered during the bottom-up analysis
Text and figures: Team of the Department of Analytical Chemistry