Research Overview

Part of the scientific work of the biophysics section is focussed on the preparation of radiolabeled agents (peptides, monoclonal antibodies) with respect to a possible application in either radiodiagnosis or radiotherapy. The resulting radiopreparations are tested for their stability and maintainance of their biological properties, such as affinity to a targeted biological structure (particularly cell receptors) in in vitro conditions. To monitor radiopreparation stability the established HPLC with radiodetection and ITLC methods are used. For the affinity studies, human cell cultures, often of tumor origin, with the relevant receptor types are used. Radioligand receptor interaction can be monitored in real-time with the employment of the LigandTracer instrument, which represents a real-time radioimmunoassay (www.ridgeview.eu). The laboratory of radiopharmaceuticals is equipped with all above stated devices. Another research direction of the Biophysics section, in collaboration with the Centre for Research and Development Teaching Hospital in Hradec Kralove, is engaged in monitoring the extent of oxidative damage to the human body. Oxidative damage to cells is one of the most common types of damage that affects all cell components. Its result are oxidative changes in the structures of lipids, proteins and DNA. In our work we focus on DNA damage. The degree of oxidative DNA damage at the level of individual cells can be determined using the so-called comet assay test. This is an important method used for the detection of single and double stranded breaks. In our research, we deal with the impact of external factors such as prevalent anesthesia and surgery, polytrauma and subsequent hospitalization in the ICU.

The physical chemistry section studies the thermodynamic characteristics of drug interactions with biological macromolecules and utilization of the interesting photophysical properties of porphyrin derivatives in cancer therapy, and as fluorescent sensors respectively, see the azaphthalocyanin research group. In cooperation with other research groups at the faculty, it determines the physicochemical characteristics of newly prepared drug candidates and develops HPLC methods for some biochemical studies. It also develops HPLC methods for studying the kinetics of degradation of dosage forms based on biodegradable copolyesters of lactic and glycolic acid.

The mathematics and statistics section is dedicated to computational pharmacy and similar research directions such as farmacometrics, systems and mathematical pharmacy and other pharmacological or biological fields based on mathematical modeling and mathematical simulation. More statistically-oriented are areas such as design of experiments or data mining, which belong to biostatistics and bioinformatics, and are often used in pharmaceutical research.

Currently offered topics of dissertations with supervisors or consultants of the department of biophysics and physical chemistry:

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