Group of Cellular and Molecular Pharmacology Charles University in Prague Faculty of Pharmacy in Hradec Kralove, Czech Republic Department of Pharmacology and Toxicology

Doc. PharmDr. Frantisek Staud, PhD Current PhD students: Mgr. Jakub Hofman Mgr. Davoud Ahmadimoghaddam Past PhD students: PharmDr. Martina Ceckova, PhD PharmDr. Antonin Libra, PhD PharmDr. Lukas Cerveny, PhD PharmDr. Zuzana Vackova, PhD PharmDr. Leos Fuksa, PhD PharmDr. Lenka Cygalova, PhD PharmDr. Lucie Svecova, PhD PharmDr. Eva Brcakova, PhD
	From left to right: Leos, Zuzana, Lukas, Martina, Petr, Frantisek, Antonin To see other, less formal photos of our group, click here.

Ongoing research projects:
Inhibition of Breast Cancer Resistance Protein (BCRP/ABCG2) by Purine Cyclin-dependent Kinase Inhibitors In Vitro and In Situ
Detoxication role of the placenta - drug transport and metabolism.
Functional evaluation of ABC efflux transporters and cationic transporters in dually perfused rat placenta and HRP-1 placental cell line.
Functional expression of ABC transporters in the BeWo cell line.
Interactions of antiepileptics with ABC transporters.
siRNA for gene silencing of selected ABC transporters.
Corticosterone metabolism in the rat placenta.
Drug inductions of ABC transporters.

Associate Professor PharmDr. Frantisek Staud, PhD

Current and past research interests:

Detoxication role of the placenta - drug transport and metabolism in umbilically/dually perfused rat placenta and placenta-derived cell lines. Drug interactions with efflux transporters and biotransformation enzymes. RNA interference for functional genomics of drug efflux transporters. Overcoming biological barriers for cancer gene therapy; application of fluorescence microscopy for tracing plasmid DNA and its expression in tumor tissues in vivo; modification of gene expression by steroids in in vitro cell cultures; targeted delivery of macromolecules in the rat in vivo; effect of radioactive labeling on pharmacokinetic analysis of macromolecules; identification of factors controlling liver uptake and hepato-biliar transfer of macromolecules; gene-delivery using cationic liposomes and polypeptides.

Curriculum Vitae:

• Education:
  December 2009 - Professorship lecture presented
  October 2002: Habilitation thesis presented ("Distribution of compounds in the body: pharmacokinetic analysis of drug transplacental passage and targeted distribution of macromolecules") at The Faculty of Pharmacy in Hradec Kralove, Charles University in Prague.
  January 1997: PhD thesis presented ("Pharmacokinetics of Placental Transfer of Drugs.") at The Faculty of Pharmacy in Hradec Kralove, Charles University in Prague.
  1994 - 1997: PhD course - Department of Pharmacology and Toxicology, Faculty of Pharmacy in Hradec Kralove, Charles University in Prague.
  1989 - 1994: Pregradual study course, Faculty of Pharmacy in Hradec Kralove, Charles University in Prague.

• Positions:
  2001 - present: Associate Professor, Dept. of Pharmacology and Toxicology, Faculty of Pharmacy, Charles University, Czech Republic
  1999 - 2001: Post-doc research fellow, School of Pharmaceutical Sciences, Manchester University, Great Britain.
  1997 - 1999: Post-doc research fellow, School of Pharmaceutical Sciences, Kyoto University, Japan.
  1994 - 1997: Research fellow, Department of Pharmacology and Toxicology, Faculty of Pharmacy in Hradec Kralove, Charles University in Prague.

• Teaching activities:
  Lectures and seminars in pharmacology to under(post)graduate students of Pharmacy and Bioanalytics.
  Supervising PhD and diploma thesis students at the Department of Pharmacology and Toxicology.
  

Grants:

1. Interactions of Breast Cancer Resistance Protein (BCRP/ABCG2) and Purine Cyclin-dependent Kinase Inhibitors In Vitro and In Situ.
2. Localization and function of Organic Cation Transporter 3 (OTC3) in the Rat Placenta
3. In-vitro study of molecular-genetic cause of cell resistance against selected drugs.
4. Investigation of drug efflux transporters using RNA interference and transfected cell lines
5. Cytochrome P450 3A4 and multidrug resistance proteins MDR1 and MRP2 as main cause of drug interaction during elimination of drugs from organism.
6. RNA interference in transepithelial transport studies
7. Study of interactions of antiepileptic drugs with efflux transporters (BCRP, MRP2 and P-glycoprotein) in vitro.
8. Visualization of placental membrane transporters using fluorescence microscopy.
9. Building laboratories of molecular pharmacology.
   

Non-research activities: When not in the lab or writing up yet another grant application, I am busy to entertain my family.
Life is too short to live it only once; so I live it twice. Next to science, I love to travel the world as a photographer for magazines and agents, see www.photostaud.com and www.phototravels.net

List of publications:

• STAUD, F.; CECKOVA, M.; MICUDA, S.; PAVEK, P. Expression and function of p-glycoprotein in normal tissues: effect on pharmacokinetics. Methods Mol Biol, 2010, vol. 596, no. s. 199-222.
• FUKSA, L.; BRCAKOVA, E.; KOLOUCHOVA, G.; HIRSOVA, P.; HROCH, M.; CERMANOVA, J.; STAUD, F.; MICUDA, S. Dexamethasone reduces methotrexate biliary elimination and potentiates its hepatotoxicity in rats. Toxicology, 2010, vol. 267, no. 1-3, s. 165-171.
• CERMANOVA, J.; FUKSA, L.; BRCAKOVA, E.; HROCH, M.; KUCERA, O.; KOLOUCHOVA, G.; HIRSOVA, P.; MALAKOVA, J.; STAUD, F.; MARTINKOVA, J.; CERVINKOVA, Z.; MICUDA, S. Up-regulation of renal Mdr1 and Mrp2 transporters during amiodarone pretreatment in rats. Pharmacol Res, 2010, vol. 61, no. 2, s. 129-135.
• VACKOVA, Z.; VAGNEROVA, K.; LIBRA, A.; MIKSIK, I.; PACHA, J.; STAUD, F. Dexamethasone and betamethasone administration during pregnancy affects expression and function of 11 beta-hydroxysteroid dehydrogenase type 2 in the rat placenta. Reprod Toxicol, 2009, vol. 28, no. 1, s. 46-51.
• PAVEK, P.; CECKOVA, M.; STAUD, F. Variation of drug kinetics in pregnancy. Curr Drug Metab, 2009, vol. 10, no. 5, s. 520-529.
• CYGALOVA, L. H.; HOFMAN, J.; CECKOVA, M.; STAUD, F. Transplacental pharmacokinetics of glyburide, rhodamine 123, and BODIPY FL prazosin: effect of drug efflux transporters and lipid solubility. J Pharmacol Exp Ther, 2009, vol. 331, no. 3, s. 1118-1125.
• BRCAKOVA, E.; FUKSA, L.; CERMANOVA, J.; KOLOUCHOVA, G.; HROCH, M.; HIRSOVA, P.; MARTINKOVA, J.; STAUD, F.; MICUDA, S. Alteration of methotrexate biliary and renal elimination during extrahepatic and intrahepatic cholestasis in rats. Biol Pharm Bull, 2009, vol. 32, no. 12, s. 1978-1985.
• VAGNEROVA, K.; VACKOVA, Z.; KLUSONOVA, P.; STAUD, F.; KOPECKY, M.; ERGANG, P.; MIKSIK, I.; PACHA, J. Reciprocal changes in maternal and fetal metabolism of corticosterone in rat during gestation. Reprod Sci, 2008, vol. 15, no. 9, s. 921-931.
• SVECOVA, L.; VRZAL, R.; BURYSEK, L.; ANZENBACHEROVA, E.; CERVENY, L.; GRIM, J.; TREJTNAR, F.; KUNES, J.; POUR, M.; STAUD, F.; ANZENBACHER, P.; DVORAK, Z.; PAVEK, P. Azole antimycotics differentially affect rifampicin-induced pregnane X receptor-mediated CYP3A4 gene expression. Drug Metab Dispos, 2008, vol. 36, no. 2, s. 339-348.
• MICUDA, S.; FUKSA, L.; BRCAKOVA, E.; OSTERREICHER, J.; CERMANOVA, J.; CIBICEK, N.; MOKRY, J.; STAUD, F.; MARTINKOVA, J. Zonation of multidrug resistance-associated protein 2 in rat liver after induction with dexamethasone. J Gastroenterol Hepatol, 2008, vol. 23, no. 7 Pt 2, s. e225-230.
• MICUDA, S.; BRCAKOVA, E.; FUKSA, L.; CERMANOVA, J.; OSTERREICHER, J.; HROCH, M.; MOKRY, J.; PEJCHAL, J.; MARTINKOVA, J.; STAUD, F. P-glycoprotein function and expression during obstructive cholestasis in rats. Eur J Gastroenterol Hepatol, 2008, vol. 20, no. 5, s. 404-412.
• FUKSA, L.; BRCAKOVA, E.; CERMANOVA, J.; HROCH, M.; CHLADEK, J.; KOLOUCHOVA, G.; MALAKOVA, J.; MARTINKOVA, J.; STAUD, F.; MICUDA, S. Amiodarone modulates pharmacokinetics of low-dose methotrexate in rats. Biopharm Drug Dispos, 2008, vol. 29, no. 5, s. 289-299.
• CYGALOVA, L.; CECKOVA, M.; PAVEK, P.; STAUD, F. Role of breast cancer resistance protein (Bcrp/Abcg2) in fetal protection during gestation in rat. Toxicol Lett, 2008, vol. 178, no. 3, s. 176-180.
• CECKOVA, M.; VACKOVA, Z.; RADILOVA, H.; LIBRA, A.; BUNCEK, M.; STAUD, F. Effect of ABCG2 on cytotoxicity of platinum drugs: Interference of EGFP. Toxicol In Vitro, 2008, vol. 22, no. 8, s. 1846-1852.
• VINSOVA, J.; IMRAMOVSKY, A.; BUCHTA, V.; CECKOVA, M.; DOLEZAL, M.; STAUD, F.; JAMPILEK, J.; KAUSTOVA, J. Salicylanilide acetates: synthesis and antibacterial evaluation. Molecules, 2007, vol. 12, no. 1, s. 1-12.
• VACKOVA, Z.; LIBRA, A.; CECKOVA, M.; PAVEK, P.; STAUD, F. Effect of dexamethasone and betamethasone on 11beta-hydroxysteroid dehydrogenase type 2 expression in human choriocarcinoma Jeg3 cells. Biomed Pap, 2007, vol. 151, no. s. 95-97.
• STAUD, F. Molekulární farmakologie látek stimulujících erytropoézu. Remedia, 2007, vol. 17, no. s. 485-494.
• SEDLAK, M.; PRAVDA, M.; STAUD, F.; KUBICOVA, L.; TYCOVA, K.; VENTURA, K. Synthesis of pH-sensitive amphotericin B-poly(ethylene glycol) conjugates and study of their controlled release in vitro. Bioorg Med Chem, 2007, vol. 15, no. 12, s. 4069-4076.
• PAVEK, P.; CERVENY, L.; SVECOVA, L.; BRYSCH, M.; LIBRA, A.; VRZAL, R.; NACHTIGAL, P.; STAUD, F.; ULRICHOVA, J.; FENDRICH, Z.; DVORAK, Z. Examination of Glucocorticoid receptor alpha-mediated transcriptional regulation of P-glycoprotein, CYP3A4, and CYP2C9 genes in placental trophoblast cell lines. Placenta, 2007, vol. 28, no. 10, s. 1004-1011.
• MICUDA, S.; FUKSA, L.; MUNDLOVA, L.; OSTERREICHER, J.; MOKRY, J.; CERMANOVA, J.; BRCAKOVA, E.; STAUD, F.; POKORNA, P.; MARTINKOVA, J. Morphological and functional changes in p-glycoprotein during dexamethasone-induced hepatomegaly. Clin Exp Pharmacol Physiol, 2007, vol. 34, no. 4, s. 296-303.
• CERVENY, L.; SVECOVA, L.; ANZENBACHEROVA, E.; VRZAL, R.; STAUD, F.; DVORAK, Z.; ULRICHOVA, J.; ANZENBACHER, P.; PAVEK, P. Valproic acid induces CYP3A4 and MDR1 gene expression by activation of constitutive androstane receptor and pregnane X receptor pathways. Drug Metab Dispos, 2007, vol. 35, no. 7, s. 1032-1041.
• BREMER, S.; BRITTEBO, E.; DENCKER, L.; KNUDSEN, L. E.; MATHISIEN, L.; OLOVSSON, M.; PAZOS, P.; PELLIZZER, C.; PAULESU, L. R.; SCHAEFER, W.; SCHWARZ, M.; STAUD, F.; STAVREUS-EVERS, A.; VAHANKANGAS, K. In vitro tests for detecting chemicals affecting the embryo implantation process. The report and recommendations of ECVAM workshop 62 -- a strategic workshop of the EU ReProTect project. Altern Lab Anim, 2007, vol. 35, no. 4, s. 421-439.
• VINSOVA, J.; CERMAKOVA, K.; TOMECKOVA, A.; CECKOVA, M.; JAMPILEK, J.; CERMAK, P.; KUNES, J.; DOLEZAL, M.; STAUD, F. Synthesis and antimicrobial evaluation of new 2-substituted 5,7-di-tert-butylbenzoxazoles. Bioorg Med Chem, 2006, vol. 14, no. 17, s. 5850-5865.
• STAUD, F.; VACKOVA, Z.; POSPECHOVA, K.; PAVEK, P.; CECKOVA, M.; LIBRA, A.; CYGALOVA, L.; NACHTIGAL, P.; FENDRICH, Z. Expression and Transport Activity of Breast Cancer Resistance Protein (Bcrp/Abcg2) in Dually Perfused Rat Placenta and HRP-1 Cell Line. J Pharmacol Exp Ther, 2006, vol. 319, no. 1, s. 53-62.
• STAUD, F.; MAZANCOVA, K.; MIKSIK, I.; PAVEK, P.; FENDRICH, Z.; PACHA, J. Corticosterone transfer and metabolism in the dually perfused rat placenta: effect of 11beta-hydroxysteroid dehydrogenase type 2. Placenta, 2006, vol. 27, no. 2-3, s. 171-180.
• LIBRA, A.; FERNETTI, C.; LORUSSO, V.; VISIGALLI, M.; ANELLI, P. L.; STAUD, F.; TIRIBELLI, C.; PASCOLO, L. Molecular determinants in the transport of a bile Acid-derived diagnostic agent in tumoral and nontumoral cell lines of human liver. J Pharmacol Exp Ther, 2006, vol. 319, no. 2, s. 809-817.
• FUKSA, L.; MICUDA, S.; CERMANOVA, J.; BRCAKOVÁ, E.; STAUD, F. Physiological role of MRP2 (Fyziologická funkce MRP2). Cesk Fysiol, 2006, vol. 55, no. s. 9-16.
• CERVENY, L.; PAVEK, P.; MALAKOVA, J.; STAUD, F.; FENDRICH, Z. Lack of Interactions between Breast Cancer Resistance Protein (BCRP/ABCG2) and Selected Antiepileptic Agents. Epilepsia, 2006, vol. 47, no. 3, s. 461-468.
• CECKOVA-NOVOTNA, M.; PAVEK, P.; STAUD, F. P-glycoprotein in the placenta: Expression, localization, regulation and function. Reprod Toxicol, 2006, vol. 22, no. 3, s. 400-410.
• CECKOVA, M.; LIBRA, A.; PAVEK, P.; NACHTIGAL, P.; BRABEC, M.; FUCHS, R.; STAUD, F. Expression and functional activity of breast cancer resistance protein (bcrp, abcg2) transporter in the human choriocarcinoma cell line bewo. Clin Exp Pharmacol Physiol, 2006, vol. 33, no. 1-2, s. 58-65.
• STAUD, F.; PAVEK, P. Breast cancer resistance protein (BCRP/ABCG2). Int J Biochem Cell Biol, 2005, vol. 37, no. 4, s. 720-725.
• PAVEK, P.; CERVENY, L.; MICUDA, S.; STAUD, F.; NOVOTNA-CECKOVA, M.; FENDRICH, Z. Nuklearni receptory: xenosenzory zprostredkujici odpoved organismu na xenobiotika a pricina nekterych lekovych interakci. Remedia, 2005, vol. 15, no. s. 380-383.
• LIBRA, A.; FERNETTI, C.; VISIGALLI, M.; ANELLI, P. L.; LORUSSO, V.; STAUD, F.; TIRIBELLI, C.; PASCOLO, L. Transport mechanisms responsible for the hepatic uptake of the bile acid derived diagnostic agent B22956: an 'in vitro' study FEBS JOURNAL, 2005, vol. 272, no. s. 226-227.
• NOVOTNA, M.; LIBRA, A.; KOPECKY, M.; PAVEK, P.; FENDRICH, Z.; SEMECKY, V.; STAUD, F. P-glycoprotein expression and distribution in the rat placenta during pregnancy. Reprod Toxicol, 2004, vol. 18, no. 6, s. 785-792.
• NISHIKAWA, M.; TAMADA, A.; HYOUDOU, K.; UMEYAMA, Y.; TAKAHASHI, Y.; KOBAYASHI, Y.; KUMAI, H.; ISHIDA, E.; STAUD, F.; YABE, Y.; TAKAKURA, Y.; YAMASHITA, F.; HASHIDA, M. Inhibition of experimental hepatic metastasis by targeted delivery of catalase in mice. Clin Exp Metastasis, 2004, vol. 21, no. 3, s. 213-221.
• PAVEK, P.; STAUD, F.; FENDRICH, Z.; SKLENAROVA, H.; LIBRA, A.; NOVOTNA, M.; KOPECKY, M.; NOBILIS, M.; SEMECKY, V. Examination of the functional activity of P-glycoprotein in the rat placental barrier using rhodamine 123. J Pharmacol Exp Ther, 2003, vol. 305, no. 3, s. 1239-1250.
• FENDRICH, Z.; STAUD, F. Transdermální náplasti - nová metoda kombinované hormonální kontracepce. Remedia, 2003, vol. 5, no. 13, s. 339-342.
• SKLENAROVA, H.; PAVEK, P.; SATINSKY, D.; SOLICH, P.; KARLICEK, R.; STAUD, F.; FENDRICH, Z. Determination of rhodamine 123 by sequential injection technique for pharmacokinetic studies in the rat placenta. Talanta, 2002, vol. 58, no. s. 1145-1149.
• PAVEK, P.; FENDRICH, Z.; STAUD, F. [Physiologic function of P-glycoprotein]. Cesk Fysiol, 2002, vol. 51, no. 3, s. 99-107.
• FENDRICH, Z.; STAUD, F. Norgestimat - peroralni kontraceptivum. Remedia, 2002, vol. 5, no. 12, s. 343-347.
• PAVEK, P.; FENDRICH, Z.; STAUD, F.; MALAKOVA, J.; BROZMANOVA, H.; LAZNICEK, M.; SEMECKY, V.; GRUNDMANN, M.; PALICKA, V. Influence of P-glycoprotein on the transplacental passage of cyclosporine. J Pharm Sci, 2001, vol. 90, no. 10, s. 1583-1592.
• STAUD, F.; NISHIKAWA, M.; TAKAKURA, Y.; HASHIDA, M. Liver uptake and hepato-biliary transfer of galactosylated proteins in rats are determined by the extent of galactosylation. Biochim Biophys Acta, 1999, vol. 1427, no. 2, s. 183-192.
• STAUD, F.; NISHIKAWA, M.; MORIMOTO, K.; TAKAKURA, Y.; HASHIDA, M. Disposition of radioactivity after injection of liver-targeted proteins labeled with 111In or 125I. Effect of labeling on distribution and excretion of radioactivity in rats. J Pharm Sci, 1999, vol. 88, no. 6, s. 577-585.
• PAVEK, P.; STAUD, F.; FENDRICH, Z.; MALAKOVA, J.; PALICKA, V. Transfer of clorazepate and nordiazepam across the umbilically perfused rat term placenta in situ: Comparison with flunitrazepam and diazepam. Trophoblast Res, 1999, vol. 13, no. s. 329-342.
• NISHIKAWA, M.; STAUD, F.; TAKEMURA, S.; TAKAKURA, Y.; HASHIDA, M. Pharmacokinetic evaluation of biodistribution data obtained with radiolabeled proteins in mice. Biol Pharm Bull, 1999, vol. 22, no. 2, s. 214-218.
• STAUD, F.; MORIMOTO, K.; NISHIKAWA, M.; TAKAKURA, Y.; HASHIDA, M. Pharmacokinetic analysis of the in vivo disposition of chemically modified proteins in rats: effect of radiolabeling method. J Pharm Sci (Japan), 1998, vol. 58, no. s. 101.
• STAUD, F.; FENDRICH, Z.; KARLICEK, R.; PAVEK, P. Unidirectional transfer of D-xylose across the rat placenta. Clin Exp Pharmacol Physiol, 1998, vol. 25, no. 1, s. 54-56.
• STAUD, F.; FENDRICH, Z.; JINDROVA, O. Pharmacokinetic examination of antipyrine passage through the placenta and the small intestine in rats. Eur J Drug Metab Pharmacokinet, 1998, vol. 23, no. 2, s. 118-124.
• STAUD, F.; FENDRICH, Z.; HARTL, J.; JINDROVA, O.; LAZNICEK, M. Different transfers of N-acetyl-p-aminobenzoic acid and p-aminobenzoic acid across the placenta and the small intestine in rats. J Drug Target, 1998, vol. 5, no. 3, s. 207-213.
• STAUD, F.; FENDRICH, Z.; KOPECKA, J.; PALICKA, V. Diazepam transfer across the rat term placenta perfused in situ. Pharm Pharmacol Lett, 1997, vol. 7, no. 1, s. 1-4.