The Effect of Trichloracetic Acid on Flavonoid Production in Ononis arvensis L. Culture in vitro

Lenka Tůmová¹ (tumova@faf.cuni.cz), Eliška Petrů¹, Jana Řimáková¹, Jiří Tůma²

¹ Department of Pharmacognosy, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Czech Republic
² Department of Biology, University of Hradec Králové, Faculty of Education, Czech Republic

Summary

The tested elicitor – trichloracetic acid influenced the production of flavonoids in Ononis arvensis L. callus and suspension cultures. Maximal increase in the production of flavonoids in callus culture was recorded with the use of trichloracetic acid in a concentration of 6.12 x 10^-3 mol L^-1 24 hours after application. Flavonoid production was increased by 107 % against control. In suspension culture, the flavonoid content was enhanced in about 105 % 24 hours after treatment with 6.12 x 10^-4 mol L^-1 of trichloracetic acid.

It seams that both the callus culture and suspension culture of Ononis arvensis are sensitive to trichloracetic acid as an elicitor of flavonoid production. The changes in flavonoid production can be influenced not only by the type of elicitor and elicitation time but also by the elicitor concentration and the type of culture in vitro.