Prevention of Anthracycline Type Antiobiotic Toxicity Using New Fe-Chelating Agents. Methodological Aspects

Ladislava Schröterová¹, Eva Kvasničková¹ (kvasnice@faf.cuni.cz), Vladimír Geršl²

¹Department of Biochemical Sciences, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Czech Republic,
²Department of Pharmacology, Charles University in Prague, Faculty of Medicine in Hradec Králové, Czech Republic

Summary

In this article we have focused on optimization of experimental conditions from the point of view of animal tissues sampling, storage conditions, subsequent processing of the samples, and the determination of enzyme activities in the tissue samples. The results are important from the viewpoint of further investigation of the new lipophilic iron chelates PIH and SIH on the activity of enzymes involved in the metabolism of anthracyclines.