Testing of Potential Growth Regulators on Plant Cultures In Vitro

Jiřina Dušková¹ (duskova@faf.cuni.cz), Jaroslav Dušek²

¹Department of Pharmaceutical Botany and Ecology,
²Department of Pharmacognosy
Faculty of Pharmacy, Charles University, Hradec Králové, Czech Republic

Summary

Newly developed 3-allyl-6-nitro-2-benzothiazolinone (ANB) growth regulator was tested either added to a medium alone or in combination with kinetin (K). Lactic acid was tested at the same time. The influence of these substances on the growth, morphogennic activity and production of secondary metabolites in tissue cultures Arctostaphylos uva-ursi, Coronilla varia, Datura meteloides, Leuzea carthamoides, Rhodiola rosea and Rheum palmatum was studied. ANB (0.02; 0.0002 mg.l^-1) and both its combinations with kinetin (0.1 mg.l^-1) showed statistically significantly higher growth of fresh weight in comparison with the control only at the Leuzea carthamoides culture. The Rheum palmatum culture showed statistically significantly higher growth only at higher concentration of ANB in combination with kinetin. Lactic acid induced statistically significantly higher growths at the Datura meteloides (10.0 mg.l^-1) and Rhodiola rosea (in all tested concentrations) cultures. The influence of both regulators on morphogennic activity proved to be ineffective at the cultures we tested with the only exception of the Leuzea carthamoides culture. At this culture little deformed buds were formed due to the influence of ANB. The influence of ANB on the production of secondary metabolites proved to be positive only at the Rheum palmatum culture. When it was present, cultures contained increased amount of flavonoids and anthraquinones in the medium.