Analysis of Flavonoids I.
Isotachophoretic Properties of Quercetin

Marie Pospíšilová¹ (pospisim@faf.cuni.cz), Jiřina Spilková²

¹Department of Analytical Chemistry,
²Department of Pharmacognosy,
Faculty of Pharmacy, Charles University, Hradec Králové, Czech Republic

Summary

The system for the rapid analysis of quercetin has been established using capillary isotachophoresis. Under the conditions employed, authentic quercetin examined was clearly separated into a sharp zone. The following operational system has been used: 5 mmol l^-1 HCl + 10 mmol l^-1 Tris (pH 8.06) as LE and 10 mmol l^-1 Glycine + Ba²⁺ as TE. The effective mobility of quercetin was given. This technique seems to be useful for the determination of quercetin in the range 40 to 400 µg ml^-1 (r = 0.9996). The proposed method is acceptably time efficient and can be used for quality control of authentic quercetin in pharmaceutical analysis.