Simultaneous Determination of Cardenolides and Coumarins in the Seeds of Coronilla varia L.

Lubomír Opletal¹ (opletal@faf.cuni.cz), Karel Vokáč², Vladimír Hanuš³, Marie Sovová¹, Gerald Blunden⁴, Asmita Patel⁴, Christopher G. Dacke⁴

¹Department of Pharmaceutical Botany and Ecology, Faculty of Pharmacy, Charles University, Hradec Králové, Czech Republic
²Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague
³J. Heyrovský Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Prague
⁴School of Pharmacy and Biomedical Science, University of Portsmouth, St Michael’s Building, White Swan Road, Portsmouth, Hants PO1 2DT, UK

Summary

The paper describes a simultaneous quantitative determination of the main components of the ripe seeds of the prospective medicinal plant crown vetch (Coronilla varia L., Fabaceae) — coumarins (scopoletin, daphnoretine, umbelliferon) and cardiotonic glycosides (deglucohyrcanoside, hyrcanoside) — by HPLC and an effective procedure for isolation of cardiotonic glycosides.