Application of Flow Injection Analysis in the Analysis of Pharmaceuticals and Biologically Active Compounds

Petr Solich (solich@faf.cuni.cz),

Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Hradec Králové, Czech Republic

Summary

The present paper is an abridgement of the present author’s dissertation on the basis of which he has obtained his associate professor degree. It summarizes the author’s research on the development and application of the method of flow injection analysis (FIA) in the field of the analysis of pharmaceuticals and biologically active compounds. The individual original papers resulted from the research of the State Research Task VIII-8-2/9-1 entitled “Instrumental Methods of the Analysis of Pharmaceuticals and Biologically Significant Compounds” investigated at the Faculty of Pharmacy, Charles University, Hradec Králové, under the supervision of Prof. RNDr. R. Karlíček, DrSc., in 1985—1989, and from the present author’s research of the grants “Automated Analytical System for the Determination of Content Uniformity of Pharmaceuticals” (Charles University Grant Agency 35/1993), “Development of Flow-Injection Analysis Used for the Determination of Low Content of Drugs and Biologically Active Compounds” (Charles University Grant Agency 23/1995) and “Development of Flow Methods for Dissolution Studies of Formulations” (Charles University Grant Agency 203/1996), and recently within the framework of the author’s direct research cooperation with Prof. M. A. Koupparis at the University of Athens, Greece.

The present paper includes papers dealing with the application of FIA in a strictly pharmaceutical field, i. e. in the determination of the content of active ingredients in mass-produced pharmaceutical preparations, including both the determination of the total content and, in a number of cases, of the content uniformity as a picture of the distribution of the active ingredient in the individual tablets of the pharmaceutical preparation. It also includes papers which make use of FIA method for the analysis of biologically active compounds. The published papers contribute to a wider application of FIA method in the field of pharmaceutical analysis, which is in agreement with the trends in the world, where in recent years the number of published papers in the very field of combination of FIA method and pharmaceutical analysis has been considerably increased. It can be expected that particularly the methods making use of the flow fluorimetric detection, immobilized enzymes, preconcentration, absorption to the solid phase, and in recent years also biosensors will be applied in thi s field in an ever-increasing extent.