HPLC Monitoring of Actual Levels of Some Non-Steroidal Antirheumatics in The Blood

Jaroslav Sochor¹ (sochor@faf.cuni.cz), Jiří Klimeš¹, Jiří Sedláček²

¹Department of Pharmaceutical Chemistry and Drug Control, Faculty of Pharmacy, Charles University, Hradec Králové
²Department of Pathological Physiology, Faculty of Medicine, Charles University, Hradec Králové

Summary

Drug levels of sodium salicylate, acetylsalicylic acid, ibuprofen and kebuzone were determined in the whole blood. Simultaneously, the distribution of the above mentioned drugs between erythrocytes and plasma was observed. An HPLC method for their analysis in samples of the whole blood, erythrocytes and plasma has been developed. These samples, after their suitable treatment, were analysed on reversed-phase columns, detected at ultraviolet spectra and the antirheumatics were quantified using the internal standards. The levels of salicylic acid, acetylsalicylic acid, ibuprofen, kebuzone and their possible metabolites were investigated in laboratory rabbits in 2-hour pharmacokinetic studies.