High-pressure Liquid Chromatographic Behaviour of the Substituted 1-Phenyl-5-Mercaptotetrazoles

Jiří Gabriel¹, Naďa Zimová², Ivan Němec², Karel Waisser³ (waisser@faf.cuni.cz), Alexandr Hrabálek³

¹Microbiological Institute, Academy of Sciences of the Czech Republic, Prague
²Department of Analytical Chemistry, Faculty of Natural Science, Charles University, Prague, Czech Republic
³Department of Inorganic and Organic Chemistry, Faculty of Pharmacy, Charles University, Hradec Králové, Czech Republic

Summary

The basic chromatographic study of a new 1-phenyl-5-mercaptotetrazole derivatives was performed using a silica column with chloroform—acetonitrile (containing 1% of acetic acid) mobile phase. The optimal composition of mobile phase for 1-(4-bromophenyl)-5-mercaptotetrazole, compound with the best thyreostatic activity from other derivatives, was chloroform : acetonitrile 92.5 : 7.5. The detection limit of 1-(4-bromophenyl)-5-mercaptotetrazole, 2 ng (injection 1 µl), was established.