[^99mTc]-1,2-Diaminecyclohexane-N,N,N',N'-Tetrakis Methylenephosphonic Acid as a Potential Bone Imaging Agent:
Labelling and Distribution in Rats

Alice Lázníčková¹ (laznicko@faf.cuni.cz), František Budský², Milan Lázníček¹

¹Faculty of Pharmacy, Charles University, Hradec Králové, Czech Republic
²Nuclear Research Centre, Řež near Prague, Czech Republic

Summary

In this work the conditions for ^99mTc-labelling of two phosphonate compounds EDTMP (ethylenediamine-N,N,N',N'-tetrakis (methylene phosphonic acid)) and DCHMP (diaminecyclohexane-N,N,N',N'-tetrakis (methylene phsphonic acid)) and their biodistribution in rats were studied for sceletal scintigraphy purposes. Both ^99mTc-chelates studied cleared rapidly from the blood and non-osseous tissues and selectively localized in the bone. A significant portion of radioactivity was found also in the kidney due to their glomerular filtration from the blood. ^99mTc-DCHMP was shown as alternative bone scintigraphy agent with near biodistribution and complexing ability to other commonly used ^99mTc-bone radiopharmaceuticals.